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Objective To study on the relationship between the serum level of highsensitivity c-reaction protein (hs-CR),matrix metalloproteinase 9 (MMT-9),tissue inhibitor of metalloproteinase 1 (TIMP-1) and carotid plaque stability in elderly people.Methods According to the carotid ultrasonography examination,120 old people were divided into vulnerable plaque group (group A,n =45),stable plaque group (group B,n =41) and no plaque group (group C,n =34),and serum levels of hs-CRP,MMP-9,TIMP-1 in each group were also detected.Results The serum levels of hs-CRP and MMP-9 of unstable plaque group and stable plaque group were higher than those of non-plaque group (P ＜ 0.05).hsCRP and MMP-9 were positively correlated with carotid plaque unstability,while TIMP-1 was negatively correlated with carotid plaque unstability,hs-CRP,MMP-9 and TIMP-1 were independent risk factors for carotid plaque stability.Conclusions Serum levels of hs-CRP,MMP-9 and TIMP-1 are closely related to the stability of carotid plaque.Elevated levels of hs-CRP and MMP-9 increase the risk of carotid plaque,and elevated levels of TIMP-1 decrease the risk of carotid plaque.
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Objective The plasma levels of interleukin 6 (IL-6),high sensitive C reactive protein (hs-CRP) and the level of T cell activation were detected in the peripheral blood inflammatory factors of acquired immunodeficiency syndrome (AIDS) patients,and the relationship with opportunistic infection and prognosis was analyzed.Methods 79 human immunodeficiency virus (HIV)-positive/aids cases from May 2014 to January 2015 in first hospital of Changsha were enrolled in the study.They were divided into three groups:HIV-infected without opportunistic infections group (n =20),HIV-infected with infections group (n =43,including HIV-infected with tuberculosis group,HIV-infected with merge fungus group.HIV combined hepatitis C),death group (n =16).Serum IL-6 and the concentration of hs-CRP were detected by enzyme-linked immunosorbent assay (ELISA).Flow cytometry was used to test the CD3 + CD4 + cell count,the percentage of CD4 + CD38 + cell and CD8 + CD38 + cell in peripheral blood mononuclear cell (PBMC).Compare the differences among the three groups.Results The results showed that:the concentration of hsCRP and IL-6 in peripheral blood of HIV death group was significantly higher than that in other three groups (P ＜ 0.05).The concentration of hs-CRP and IL-6 in the peripheral blood of the HIV-infected with tuberculosis group and HIV-infected with merge fungus group were significantly higher than that in the HIV-infected without opportunistic infections group (P ＜ 0.05),and the hs-CRP in the peripheral blood of the HIV combined with the hepatitis C group was higher than that in the HIV-infected without opportunistic infections group (P ＜ 0.05).The number of CD3,CD4T lymph nodes in the death group and the combined opportunistic infection group was significantly lower than that in the HIV-infected without opportunistic infections group (P ＜ 0.05).The HIV-RNA expression in peripheral blood of the death group and the combined opportunistic infection group was significantly lower than that in the HIV-infected without opportunistic infections group (P ＜ 0.05).The expression of CD8+CD38+ on PBMC in the HIV-infected without opportunistic infections group was significantly lower than that in the death group,the tuberculosis group,the fungus group and the hepatitis C group (P ＜ 0.05).The expression of CD4+ CD38 + on PBMC in the HIV-infected without opportunistic infections group was higher than that in the death group (P ＜ 0,05).Conclusions The concentration of inflammatory cytokines (IL-6,hs-CRP) and the expression level of T cell surface CD8 + CD38 + related to immune activation were associated with opportunistic infection and prognosis of AIDS.
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Objective To investigate the application values of S100-β protein,homocysteine (Hcy) and high-sensitive C-reactive protein (hs-CRP) in the diagnosis of acute ischemic stroke(AIS).Methods A total of 73 patients with AIS were enrolled in this study.The Non-stroke patients with similar symptoms were selected as control group (n =50).The relationship between levels of S100-β protein,Hcy and hs-CRP and infarct volume was evaluated.The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic values of single and combined detection of these indicators in AIS.Results The levels of S100-β protein,Hcy and hs-CRP were higher in AIS patients than those in control group after adjusting the major vascular risk factors (P ＜ 0.05).There were significant differences in S100-β protein,Hcy and hs-CRP between different groups according to the infarct volume (P ＜ 0.05).S100-β protein,Hcy and hs-CRP positively were correlated with infarct volume (r =0.625,P =0.000;r =291,P =0.012;r =0.396,P =0.001 respectively).ROC curves showed that the diagnostic performances for AIS were as follows:S100-β protein (AUC =0.809,sensitivity:65.8％,specificity:82.0％),Hcy (AUC =0.775,sensitivity:54.8％,specificity:84.0％),hs-CRP (AUC =0.698,sensitivity:58.9％,specificity:78.0％) and Combined detection (AUC =0.878,the sensitivity:68.5 ％,specificity:94％).Conclusions S100-β protein,Hcy and hs-CRP protein can be used as biomarkers for AIS,which can help the diagnosis of AIS.S100-β protein can be used as a biomarker to evaluate the severity of AIS,Hcy and hs-CRP can also reflect the severity of AIS to some extent.Single detections of S100-β protein,Hcy and hs-CRP show limited value in the diagnosis of AIS.Combined detection gains a high specificity,but sensitivity is not very high.
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Objective To evaluate the diagnostic value of ferritin,procalcitonin (PCT) and C-reactive protein (CRP) on infectious diseases.Methods A total of 190 patients with infectious diseases in our hospital from 2012 to 2017 were enrolled and analysed retrospectively.Among them,bacterial infection was found in 130 cases (bacterial infection group),and viral infection in 60 cases (viral infection group).Serum levels of erritin,CRP,PCT in 190 patients were deteceted for further statistical analysis.Results Among the 190 cases of infectious fever,serum levels of erritin,CRP,PCT in bacterial infection group were higher than that in the viral infection group,with significant difference.The receiver operating curve (ROC) curve analysis showed that area under curve (AUC) of CRP,PCT,ferritin were 0.797,0.803,0.883.Conclusions The present study identified ferritin,PCT and CRP that should be considered in the differential diagnosis of infectious diseases,providing useful information for future diagnosis.
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Objective To investigate procalcitonin (PCT) and C reactive protein (CRP) expression in patients with puerperal infection after cesarean section and its clinical significance.Methods From January 2014 to January 2016,134 cases of cesarean section puerperal infection patients in our hospital were enrolled in observation group,according to 1∶3 matching,we selected 402 cases of cesarean section without puerperal infection as the control group.Then the levels of PCT and CRP in two groups were detected.Results CRP and PCT levels of 24 h,48 h and 72 h of the patients in the observation group were (13.16 ± 1.06)mg/L and (1.05 ±0.42)g/L,(15.15 ± 1.50)mg/L and (1.67 ±0.31)g/L,(16.01 ± 2.32) mg/L and (2.10 ± 0.92) g/L respectively,significantly higher than the control group (P ＜ 0.05);72 h CRP and PCT levels of pelvic cavity peritonitis patients were (17.13 ± 1.46)mg/L and (2.81 ± 0.97) g/L respectively,significantly higher than that in patients with vulvovaginal and cervicitis,endometritis and fallopian tube (P ＜ 0.05);72 h CRP and PCT in patients with endometritis and fallopian tube inflammation were (15.89 ± 1.06) mg/L and (2.06 ± 0.83) g/L,significantly higher than that of vaginal and Cervicitis Patients (P ＜ 0.05).Conclusions CRP and PCT increase rapidly in patients with puerperal infection after cesarean section,and there are a certain relationship with puerperal infection disease and they are of certain clinical application value.
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Objective To value different stages of chronic obstructive pulmonary disease (COPD) patients with the detection of the levels of serum thymic stromal lymphopoietin (TSLP),serum amyloid A (SAA),and C-reactive protein (CRP).Methods A total of 33 patients with COPD during and after hospitalization and 16 healthy controls were enrolled in the study from the 3rd affiliated hospital of SYSU.Differences and correlations of the level of serum TSLP,SAA and CRP were analyzed to value the veracity of those factors in different stages of COPD.Results The levels of CRP and SAA were higher in acute exacerbation of COPD (AECOPD) group than stable COPD group,TSLP was lower in the AECOPD group than the healthy control group (P ＜ 0.05).CRP had a positive correlation with SAA (correlation r =0.546,P =0.000),CRP [area under curve (AUC) =0.797] and SAA (AUC =0.815),and they were statistically significant in identity of different stages of COPD (P ＜ 0.05).Conclusions Serum TSLP level is decreased in acute exacerbation phase of COPD.CRP and SAA are increased in AECOPD.SAA is more confident in identity of different phases of COPD.
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Objective To investigate whether C-reactive protein (CRP) and neutrophil/lymphocyte ration (NLR) can predict the infection complications and predict the short-term survival rate of patients with cirrhosis.Methods Data of 208 patients with liver cirrhosis from March 2013 to March 2016 were analyzed retrospectively.The effects of CRP concentration,NLR and other clinical factors on the infection and one-month survival rate of patients with liver cirrhosis were analyzed.Results Sixty-five patients with dominant infection during hospitalization were used as the infected group,while the other 143 patients were selected as uninfected group.The levels of CRP and NLR in the infected group were significantly higher than those in the uninfected group (CRP:t =16.216,P =0.003;NLR:t =7.211,P =0.025).The results of univariate analysis showed that age,female,non-alcoholic cirrhosis,systemic inflammatory response syndrome (SIRS),high level of CRP,and high NLR value were important risk factors of infection in cirrhotic patients.In multivariate analysis,female,high level of CRP,and high NLR value were the independent predictor of infection in patients with cirrhosis.Univariate analysis showed that NLR,model for end-stage liver disease (MELD) score,and WBC count were important predictors of 1-month survival.Multivariate analysis showed that high NLR was an independent predictor of one-month survival in Child Pugh C patients.Conclusions CRP level and NLR value may be an effective diagnostic marker in patients with liver cirrhosis.The high NLR value in patients with Child-Pugh C is an independent risk factor for poor short survival.
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Objective To investigate whether C-reactive protein (CRP) and neutrophil/lymphocyte ration (NLR) can predict the infection complications and predict the short-term survival rate of patients with cirrhosis.Methods Data of 208 patients with liver cirrhosis from March 2013 to March 2016 were analyzed retrospectively.The effects of CRP concentration,NLR and other clinical factors on the infection and one-month survival rate of patients with liver cirrhosis were analyzed.Results Sixty-five patients with dominant infection during hospitalization were used as the infected group,while the other 143 patients were selected as uninfected group.The levels of CRP and NLR in the infected group were significantly higher than those in the uninfected group (CRP:t =16.216,P =0.003;NLR:t =7.211,P =0.025).The results of univariate analysis showed that age,female,non-alcoholic cirrhosis,systemic inflammatory response syndrome (SIRS),high level of CRP,and high NLR value were important risk factors of infection in cirrhotic patients.In multivariate analysis,female,high level of CRP,and high NLR value were the independent predictor of infection in patients with cirrhosis.Univariate analysis showed that NLR,model for end-stage liver disease (MELD) score,and WBC count were important predictors of 1-month survival.Multivariate analysis showed that high NLR was an independent predictor of one-month survival in Child Pugh C patients.Conclusions CRP level and NLR value may be an effective diagnostic marker in patients with liver cirrhosis.The high NLR value in patients with Child-Pugh C is an independent risk factor for poor short survival.
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Objective To investigate the serum levels of high sensitivity C-reactive protein (hsCRP),interleukin-1β (IL-1β) and lipoprotein-associated phospholipase A2 (Lp-PLA2),and investigate their clinical diagnostic value in acute coronary syndrome (ACS) patients.Methods Fifty three clinical serum samples of patients specifically diagnosed with acute coronary syndrome were collected.A total of 21 healthy subjects were enrolled as healthy controls.Serum IL-1β and Lp-PLA2 levels were determined by enzyme-linked immunosorbent assay.The concentration of hs-CRP was tested by immunoturbidimetry method.Results Compared to the healthy controls,the levels of serum hs-CRP,IL-1βand Lp-PLA2 were significantly higher in ACS and ACS subgroups (P ＜ 0.05),respectively.The level of Lp-PLA2 was gradually increased among healthy controls,angina pectoris (AP),ST-segment elevation myocardial infarction (STE-MI),non-ST-segment elevation myocardial infarction (NSTEMI) groups.The best cut-off value of hs-CRP,IL-1β and Lp-PLA2 was 7.44 mg/L,90.88 ng/L and 219.92 μg/L,respectively.The parallel test had better sensitivity (94.3％) and specificity (100％).Conclusions Serum hs-CRP,IL-1β and Lp-PLA2 play an important role in classifying the clinical types and monitoring the conditions of patients with ACS.Combination of hs-CRP,IL-1β and Lp-PLA2 is expected to be a new biomarker for ACS.
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Objective To investigate the level of the red blood cell (RBC) distribution width and other related indicators to predict the prognosis in patients with chronic heart failure elderly.Methods Retrospective analysis was performed in the patients with chronic heart failure elderly which died in our hospital (experimental group) and 100 patients were selected randomly that with heart failure elderly being improved by treatment (control group).The difference was compared in the level of the RBC distribution width (RDW),high-sensitivity C-reactive protein (hs-CRP),homocysteine (Hcy),D-dimer (DD),and amino-terminal pro-brain natriuretic peptide (NT-proBNP) in two groups.Results The level of RDW,hsCRP,Hcy,DD,and NT-proBNP in experimental group were significantly higher than that in control group (P ＜0.01).Conclusions Chronic heart failure was common clinical critically ill in the elderly.There were important clinical significance and important predictors to detect the levels of RDW,hs-CRP,Hcy,DD,and NT-proBNP in predicting prognosis and evaluating the effect of treatment.
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Objective To investigate the influence of the high-flux hemodialysis (HFD) on inflammatory factors [C-reactive protein (CRP),interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α)] and serum brain natriuretic (BNP) in patients before and after hemodialysis.Methods Fifty patients with MHD were enrolled in our hospital in 2013 and divided randomly into HD group (n =25),and HFD group (n =25).Serum CRP,IL-6,TNF-α,and BNP were measured in patients before and after the first hemodialysis session and after the treatment for 6 months later.Results Serum CRP,IL-6,and TNF-α in HD group after the first dialysis session and after the treatment for 6 months were statistically insignificant (P ＞0.05).In HD group,serum BNP was decreased after the first hemodialysis session and the treatment for 6 months later,the decreases was statistically significant (P ＜0.01 or P ＜0.05).In HFD group,serum CRP,IL-6,and TNF-α were decreased after the first treatment,the decreases were statistically insignificant (P ＞ 0.05).However,after the 6 mouths,the decreases were statistically significant (P ＜ 0.05).Serum BNP was decreased after the first hemodialysis session and the treatment for 6 months later,the decrease was statistically significant(P ＜ 0.01).Between two groups,the different time-point and the interaction of two groups and the different time-point,the discrepancy above them were statistically significant (P ＜0.01).Conclusions The high-flux hemodialysis can eliminate more inflammatory factors such as CRP,IL-6,TNF-α,and serum BNP in patients with uremia.Serum BNP Helps to adjust the dry weight in time,relieve cardiac load and reduce the incidence of heart failure.High-flux hemodialysis is one of the ways of treatment that reduce the morbidity of the heart failure.It can improve prognosis of the patients with MHD.
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Objective To investigate the levels and the abnormal incidences of apolipoprotein and other related indicators in health people in Hunan province,and to provide data for establishment of reference intervals in Hunan province.Methods The people with physical examination (n =341) were chosen from the Second Xiangya Hospital of Central South University during August 2014 ～ October 2014 (male,n =239;female,n =102) with age from 22 to 77 years old.All people were divided into 4 groups according to their age:group A aged from 22 to 42 (35.56 ± 5.39),group B aged from 43 to 48 (45.59 ± 1.59),group C aged from 49 to 55 (51.19 ± 1.81),and group D aged from 56 to 77 (63.08 ±5.84).The levels of apoAI,apoB,Lp (a),hypersensitive C-reactive protein (hs-CRP),and homocysteine (HCY) were detected with automatic biochemical analyzer,and were compared among different age groups and between male and female in each age group.Results Compared to male,the average levels of apoAI,and Lp (a) were significantly increased (P ＜0.05),and apoB,hs-CRP,and Hcy were significantly decreased (P ＜ 0.05) in the female.The levels of apoB,and hs-CRP were significantly different among 4 age groups (P ＜ 0.05).The abnormal rate in each index among 4 age groups was not significantly different (P ＞ 0.05).For groups A,B and D,the abnormal rate of Hcy was significant higher in male relative to female (P ＜ 0.05).Conclusions All the indices have significant difference between male and female (P ＜0.05).The levels of apoAI,Lp (a),hs-CRP,and Hcy do not change with the changed age.However,apoB has a certain relationship with age.The level and abnormal rate of Hcy are significantly higher in male relative to female.
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Objective To investigate the relationship between C-reactive protein (CRP) and postoperative cognitive function of female patients underwent cardiopulmonary bypass (CPB) cardiac surgery.Methods Sixty female patients were scheduled for CPB surgery from January 2012 to May 2013,aged form 35 to 60 years,mean aged (47.5 ± 12.5) years,and in state of ASAⅡ ～ Ⅲ.All patients had general anesthesia and CPB cardiac surgery.Blood samples were taken from peripheral vein before induction and 6 days after surgery.The neuropsychology assessment was measured with Wechsler Adult Intelligence Scale RC 2 + 1 (WAIS-RC) and digital span the first day preoperative,the 7th day,and the first month postoperative.The postoperative cognitive dysfunction (POCD) was judged with Newman style.Results (1) The rate of POCD of the 7th day and first month postoperatively was 50％ and 25％,respectively.(2) The Pearsong correlation showed negative correlation between CRP preoperative at the 6th day and the 7th day,and the first month postoperative WAIS-RC score(P ＜ 0.05).(3) The Pearson's figure showed negative correlation between CRP at the 6th day postoperative with digital span (P ＜ 0.05).Conclusions The ratio of POCD after CPB surgery was higher.It is instructively significant to monitor the variations of concentration of serum CRP for the selection of cognitive impairment in patients with cardiac-pulmonary bypass surgery.
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Objective To investigate the relationships between serum concentration of RBP-4 and inflammatory factors,insulin resistance in patients with gestational diabetes mellitus.Methods20 patients newly diagnosed with impaired glucose tolerance (IGT),27 patients newly diagnosed with gestational diabetes mellitus(GDM) and 50 subjects with normal glucose tolerance(NGT) were enrolled in this cross-sectional study.Additionally,another subset analysis was performed,subjects were divided on the basis of BMI into normal weight (NW) and overweight or obesity (OW/OB).Serum RBP-4 was measured with ELISA;serum concentrations of hs-CRP,free fatty acids (FFA),triglyeride(TG),total cholesterol (TC) and insulin were measured in fasting status.Insulin resistance was assessed by HOMA-IR.ResultsLn(HOMA-IR) and the concentrations of FFA,ln(hs-CRP) in IGT or in GDM were significantly higher than that of NGT(P＜0.05).In the group of OW/OB,FFA[(0.90±0.31)mmol/L vs (0.71±0.28)mmol/L,t＝2.73,P＜0.05)],ln(hs-CRP)(0.62±1.00 vs -0.17±1.07,t＝2.90,P＜0.05),ln(HOMA-IR),lnRBP(0.56±0.27 vs 0.30±0.14,t＝2.86,P＜0.05)were significantly higher than that of NW.In the group of GDM,lnRBP-4 showed a positive relationship with FFA (r＝0.231,P＜0.05)and ln(hs-CRP) (r＝0.237,P＜0.05).There was a positive relationship between lnRBP-4 and FFA(r＝0.371,P＜0.05) or ln(hs-CRP)(r＝0.247,P＜0.05) in the group of OW/OB,but no association in the group of NGT,IGT or NW.There was no relationship between lnRBP-4 and ln(HOMA-IR) in IGT,GDM and NW,while in the group of NGT and OW/OB,lnRBP-4 showed a positive relationship with ln(HOMA-IR) (r＝0.276,0.290,P＜0.05).In a multiple linear regression analysis,ln(hs-CRP),FFA or ln(HOMA-IR) was not the independent factor of lnRBP-4 in any group.ConclusionsIn patients with gestational diabetes mellitus,lnRBP-4 may be a new marker of inflammation.
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Objective To investigate the relationships between serum concentration of RBP-4 and the parameters of inflammatory, lipid metabolism, insulin resistance in patients with T2DM. Methods 71 patients newly diagnosed with T2DM and 34 subjects with normal glucose tolerance (NGT) were enrolled in this cross-sectional study. Additionally, another subset analysis was performed. Subjects were divided into normal weight (NW) and overweight or obesity (OW/OB) group on the basis of BMI. Serum RBP-4 was measured with ELISA. Serum concentrations of hs-CRP, free fatty acids (FFA), triglyceride (TG), total cholesterol (TC) and insulin were measured in fasting status. Insulin resistance was assessed by HOMA-IR. Results Ln (HOMA-IR) and the concentrations of TG, FFA, In(hs-CRP) in T2DM were significant-ly higher than that of NGR group[1.20±0.38 vs 0.76±0.34,(2.74±2. 20)mmol/L vs (1.88±1.41),(0. 80±0. 29)mmol/L vs (0.61±0.22)retool/L,0.62±1.00 vs -0. 17±1.07] . InRBP-4 showed a positive relationship with In (HOMA-IR) ( r =0. 382, P <0. 05), but no association with TC, TG, FFA and In(hs-CRP). In the group of T2DM, InRBP-4 showed a positive relationship with FFA ( r =0. 242, P <0.05) and In (hs-CRP) ( r =0.346, P <0.01), but no association with TC, TG and In (HOMA-IR). There were no relationships between InRBP-4 and In (HOMA-IR), TC, TG, FFA and In (hs-CRP)in NW, while in OW/OB group, InRBP-4 showed a positive relationship with In (HOMA-IR) ( r =0. 290,P < 0. 05 ) and In (hs-CRP) ( r = 0. 295, P <0.05 ), but no association with TC, TG and FFA. In a mul-tiple linear regression analysis, In( hs-CRP), TC, TG, FFA or In(HOMA-IR) was not the independent de-terminant of InRBP-4 in any group. Conclusion In patients with T2DM, InRBP-4 is positively correlatedwith In (hs-CRP) and FFA, and it may be a new marker of inflammation.
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Objective To investigate the serum level of visfatin, C-reactive protein (CRP) and monocyte chemoattractant protein-1 (MCP-1) in patients with polycystic ovarian syndrome (PCOS), and provide the basis for understanding the pathogenesis of PCOS. Meth-ods 94 patients with PCOS and 100 healthy subjects were divided into 4 subgroups according to their body mass index(BMI), including obese PCOS subgroup(n = 52), non - obese PCOS subgroup (n = 42), obese healthy subject subgroup (n = 43) and non - obese healthy subject (n = 57). Serum visfatin, CRP, MCP-1, sex hormone levels and metabolic parameters were measured by enzyme-linked immu-nosorbent assay (ELISA), automatic chemistry analyzer or chemiluminescent immunoassay. Results Serum levels of testosterone (T), lu-teiizing hormone (LH) and prolactin (PRL) in the PCOS group were significantly higher than those in healthy subjects group(P <0.05～0.01), and follicle-stimulating hormone (FSH) levels were significantly decreased in PCOS group(P<0.01). Serum levels of Visfatin, CRP, MCP-1,fasting insulin(Fins) and insulin resistance homa model (HOMA-IR) in the obese or non - obese PCOS subgroup were signif-icantly increased than that in the obese or non - obese healthy subjects subgroup respectively (P<0.01). Pearson correlation analysis showed that the visfatin, CRP and MCP-1 levels were positively related to BMI, FINS and HOMA-IR(r=0.323～0.675, P<0.01). Par-tial correlation showed that serum visfatin levels were correlated with HOMA-IR(r=0.491, P<0.01), and serum MCP-1 levels were cor-related with LH (r=0.267, P<0.05) in the PCOS group. Conclusion The patients with PCOS had higher visfatin, CRP and MCP-1 lev-els, and visfatin levels were positively correlated with insulin resistance. Obesity were involved in the chronic inflammation course in patients with PCOS.